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Research protocols and reported user experiences most commonly place the injection 30 to 60 minutes before the target sleep time, aligning with DSIP's short plasma half-life of roughly 30 minutes after IV administration

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While glia-derived glutamine provides 50–60% of a glutamate neurotransmitter, when the pathway is blocked with MeAIB, excitatory transmission abruptly decreases but rapidly recovers, consistent with endogenous neuronal sources of glutamate neurotransmitter (15)