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Its amino acid sequence comprises 43 amino acids: Sequence: Ac-Ser-Asp-Lys-Pro-Asp-Met-Ala-Glu-Ile-Glu-Lys-Phe-Asp-Lys-Ser-Lys-Leu-Lys-Lys-Thr-Glu-Thr-Gln-Glu-Lys-Asn-Pro-Leu-Pro-Ser-Lys-Glu-Thr-Ile-Glu-Gln-Glu-Lys-Gln-Ala-Gly-Glu-Ser Physicochemical properties: Molecular mass: ~4963 Da Empirical formula: C212H350N56O78S Solubility: high in water and buffer solutions with pH 6-8 Stability: stable at 2-8°C pKa: approximately 10.5 (due to the presence of amine groups) TB-500 exhibits a characteristic ability to bind actin – a structural protein of the cellular cytoskeleton – through the actin-binding domain located in its N-terminal part

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Baughman, J