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Where BPC-157 and TB-500 act primarily through actin/cytoskeletal and growth-factor pathways, GHK-Cu's mechanism is dominated by transcriptional reprogramming via copper-dependent gene regulation, making it a complementary research tool when investigators wish to dissect transcription-driven from structural-protein-driven repair phenotypes

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Following this, they were washed three times with PBST (1X PBS and 0.05% Tween-20), then incubated with mouse IgG FITC conjugated secondary antibody (Invitrogen)